Open Neuroimaging Framework

Anatomical
Fiducials Validator

Train and validate the placement of 32 neuroanatomical landmarks across human and non-human primate brain templates. Built for reproducible science.

Launch Validator
32
Landmarks
21
Templates
2
Species
Open access

Anatomical Fiducials (AFIDs) is an open framework for evaluating correspondence in brain images and teaching neuroanatomy through precisely defined landmark placement. Researchers and trainees place 32 landmarks in 3D Slicer, export the markup file, and upload here to receive instant, detailed placement feedback.

The protocol spans human and non-human primate brain templates, making it a cross-species tool for image registration quality control and neuroanatomy training.

AFIDs placement walkthrough in 3D Slicer

01
Place

Open 3D Slicer and follow the AFIDs protocol to place 32 anatomical landmarks on your brain image.

02
Export

Save your markup file from 3D Slicer as .fcsv or .json.

03
Validate

Upload here. Get per-landmark distance, directional error, and a regional error profile — instantly.

🧠
Regional Error Profile

Radar chart of mean placement error across 8 neuroanatomical regions — see where your accuracy breaks down at a glance.

🧭
Directional Feedback

Each landmark error decomposed into anatomical axes — "2.1mm posterior, 0.8mm superior" — immediately actionable.

📊
Session Dashboard

Mean ± SD error, best and worst landmark, and how many placements fall within 1mm and 2mm of the template.

🐒
Multi-Species

Validate against human and macaque templates. NMT, D99, INIA19, Yerkes19, MacaqueMNI all supported.

🗄️
Data Contribution

Log in with ORCID and contribute your placements to the growing community dataset.

🤖
AI-Guided Learning

New: place landmarks directly in-browser on the MNI152 template with a real-time AI tutor that gives anatomical feedback after every placement. Try it →

🔓
Open Framework

GPL-licensed, community driven, and built for reproducible neuroimaging research.

🤖 New

Guided Learning Mode

Not sure where to start? The interactive learning mode loads the MNI152NLin2009cAsym T1w template directly in your browser and walks you through all 32 landmarks one by one. After each placement, an AI tutor gives you immediate anatomical feedback — what structure you likely clicked, how to visually identify the correct target, and how close you were. No software to install.

Try Guided Learning
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Human
15 templates
Agile12v2016 BigBrain Colin27 MNI152Lin MNI152NLin2009bAsym MNI152NLin2009bSym MNI152NLin2009cAsym MNI152NLin2009cSym MNI152NLin6Asym MNI152NLin6Sym MNI2009cAsym MNI305 OASIS30ANTs PD25 fsaverage
🐒
Macaca
6 templates
D99 INIA19 MacaqueMNI NMTv1.3 NMTv2.0asym Yerkes19

Ready to validate your fiducial placements?

Open Validator